Detecting genetically modified fibres according to ISO/TS 5354-2:2024

PCR CFX96 – BioRad
gmo-cotton
Testing lab
Microbiology
Testing standard

ISO/TS 5354-2:2024

PCR

Polymerase chain reaction (PCR) is a method widely used in molecular biology to make several copies of a specific DNA segment. Using PCR, copies of DNA sequences are exponentially amplified to generate thousands to millions of more copies of that particular DNA segment.

With classical methods it is not possible to distinguish between ordinary cotton and genetically modified cotton because the difference can only be found in the DNA structure of the fibre. The same methods are also not suitable, for example, to reliably demonstrate the difference between flax and hemp. Methods based on the PCR technique (polymerase chain reaction) have already shown that they can differentiate between wool, cashmere, yak wool and their blends.

The lack of a reliable testing method opens the door to numerous fraudulent practices and without a solid method, it is impossible to to sell genetically modified cotton as ordinary or even organic cotton. The flax industry is also confronted with the practice of selling the much cheaper hemp fibres as flax fibre.

Centexbel solves this problem by using the PCR technique (polymerase chain reaction) which has very specific can detect DNA sequences. We do this in two steps:

  1. complete DNA extraction of plant samples and finished textiles
  2. amplification by PCR of certain sequences from samples containing species of interest
bio-rad-CFX96-opstellingGH
PCR CFX96 – BioRad

Principle

This appliance to amplify certain DNA sequences allows to precisely determine the biological origins of fibres and to ascertain whether a fibre has been genetically engineered or not

The DNA analysis is used to identify natural (vegetal and animal) fibres (hemp, flax, wool) and offers the industry a reliable and transparent method to detect fraud.

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singleplex or multiplex reactions.

 

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